基于静电纺丝技术的SERS基底制备表征及应用

摘要表面增强拉曼光谱（Surface-enhanced Raman spectroscopy, SERS）由于其对环境中痕量以及超痕量污染物的检测具有快速、灵敏、无损等特点，近年来备受关注。利用静电纺丝装置可制备表面增强拉曼散射所需的孔隙率高、比表面积大、长径比大、纤维均一性以及精细程度高的基底。本文以聚乙烯醇(polyvinyl alcohol, PVA)为纺丝原料，制备出负载了纳米银的柔性银基 SERS 基底。采用透射电子显微镜（Transmission ElectronMicroscope, TEM）、扫描电子显微镜（Scanning Electron Microscope, SEM）、紫外-可见分光光度计（UV-Visible Spectrophotometer, UV-vis）、拉曼光谱仪（Raman Spectrometer, Raman）等对其结构进行表征，并以亚甲基蓝（Methylene blue, MB）作为探针分子，对制备的SERS基底进行了研究。结果表明，制备的SERS 基底对探针分子的响应具有灵敏度高、准确、便捷的特点，优化条件后，浓度为 3.34×10-3mol/L 的银胶对亚甲基蓝的检测限达到1×10-5mol/L，增强因子高达 7.8×106。42429
毕业论文关键词表面增强拉曼柔性基底纳米金属增强因子•
Title The preparation, characterization and application of SERS substrate based on the electrospinning technique
Abstract SERS (Surface-enhanced Raman spectroscopy, SERS) has drawn much attention in recent yearsin the environment due to its detection of trace and ultra-trace contaminants rapid, sensitive,non-destructive characteristics. Electrostatic spinning equipment may be used for preparationsurface-enhanced Raman scattering substrate for the fabricated fibers could have high porosity,large surface area, aspect ratio, high uniformity and fineness. In this paper, polyvinyl alcohol(polyvinyl alcohol, PVA) was chosen for preparing flexible SERS substrate with nanosilverloaded. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), UV -visible spectrophotometer (UV-vis), Raman spectroscopy (Raman), etc. were used tocharacterize their structures with methylene blue (MB) as a probe molecule. The results showedthat, our prepared SERS substrate has good response to the probe molecules with highsensitivity, accuracy, and convenient features, in optimized conditions, with 3.34×10-3mol/Lsilver loading, of methylene blue plastic detection limit of 1×10- 5mol / L, and the enhancementfactor could up to 7.8×106.
Keywords Surface-enhanced Raman scattering (SERS) flexible substrateselectrostatic spinning silver nanoparticles

1引言1

1.1研究背景.1

1.2表面增强拉曼散射1

1.3静电纺丝技术2

1.4课题研究意义及内容4

2实验部分6

2.1实验仪器及药品6

2.2纺丝液的制备7

2.3表征方法.8

3实验结果9

3.1扫描电子显微镜分析9

3.2紫外可见光谱分析18

3.3透射电子显微镜分析18

3.4Raman分析23

结论与展望31

致谢32

参考文献33
1 引言1.1 研究背景伴随着人口膨胀和经济高速发展的一个不容忽视的问题就是当今亟待解决的环境问题。解决问题的第一步就是及时快捷地发现问题，表面增强拉曼由于对样品不进行直接接触、所需样品少、对样品不造成破坏、达到单分子级别的检测等特性，在环境中对痕量以及超痕量的污染物监测具有很强的能力，在检测痕量农药残余、多环芳烃和环境病原微生物等方面大放异彩[1]。近年来，越来越多的研究聚焦于将纳米材料用作表面增强拉曼基底。纳米材料作为这几十年的新兴材料一直备受关注，它的尺寸通常在1 ~ 100 nm，随着纳米材料的尺寸特别性而来的是它独特的界面效应、小尺寸效应以及量子效应等[2]。静电纺丝技术是利用高压静电来制备超薄纳米纤维的一种方法，此种方法具有高效、便捷、实用等的显著优点而备受推崇。基于静电纺丝技术的SERS基底制备表征及应用:http://www.751com.cn/huaxue/lunwen_42860.html