钙调素类似蛋白CML25-like调控花和果实发育的功能分析

摘要：本实验以CML25-like转基因黄瓜为实验材料，通过统计、分析对比T3代转基因黄瓜及对照‘长春密刺’黄瓜在不同处理不同环境下的开花结果情况，以明确CML25-like基因在黄瓜花形成和果实发育过程中的具体功能。通过在实验室内的恒温光照培养箱进行的夹花预实验及乙烯处理结果表示，150mg/L乙烯利处理对黄瓜雌花性别分化无显著作用，但却可以提升黄瓜单性结实率。转基因黄瓜第一雌花节位、总节数、总雌花数皆大于普通黄瓜。同时田间实验中结果表明，T3代转基因黄瓜单性结实率高于对照普通‘长春密刺’黄瓜，而果长、果周长等方面前者也优于后者。可以在一定程度上验证钙调素类似蛋白CML25-like具有可以调控黄瓜的花和果实的功能。29035
关键词：类钙调素；转基因黄瓜；单性结实
The Functional Analysis of CML25-like Controls the Flowers and Fruits Development
Abstract：CML25-like transgenic cucumber lines were investigated in this study. For revealing the function of CML25 involved in flower formation and fruit development, the flower types and parthenocarpic rate of CML25-like CCMC (‘Changchunmici’) was analyzed and compared. Flower trapping experiment and ethylene treatment were conducted in laboratory Constant light incubator. It was showed that 150mg/L ethylene treatment can’t promote the transformation from the sex of the cucumber flower,but it can improve the cucumber parthenocarpic rate . The first initiation of female flower is earlier and the number of the total female flower is larger in transgenic cucumber by comparing with the control plants. Both the laboratory and field experiment suggested that parthenocarpic rate of the T3 generation of transgenic cucumber is much higher than control cucumber, and fruit length, circumference were also better than the latter. Based on these findings we speculated that CML25 - like protein has the ability to regulate the flower and the fruit of the cucumber in some extent.
Key words: calmodulin; transgenic cucumber; parthenocarpy
目录
摘要1
关键词1
Abstract1
Key words1
引言1
1 材料与方法2
1．1 实验材料的选择 3
1. 2 前期实验准备 3
1. 3 对CML25-like基因调控黄瓜植株生长的探究3
1. 4 乙烯处理对CML25-like基因调控黄瓜中雌花分化的探究4
1. 5 对CML25-like基因调控黄瓜单性结实的探究4
1. 5. 1 光照培养箱实验4
1. 5. 2 田间实验4
1. 6 对CML25-like基因调控黄瓜果实大小的探究4
1．7 数据处理及分析方法 4
2 结果与分析 5
2. 1 CML25-like对黄瓜植株生长的影响 5
2. 2 乙烯处理对CML25-like过量表达转基因黄瓜中雌花分化的影响 5
2. 3 CML25-like对黄瓜单性结实的影响 6
2. 3. 1 光照培养箱实验结果分析 6
2. 3. 2 田间实验结果分析 7
2. 4 CML25-like对黄瓜果实大小的影响分析 8
3 讨论 8
致谢 9
参考文献10
附图1 11附图2 12
附图3 13
钙调素类似蛋白CML25-like调控花和果实发育的功能分析
引言：黄瓜(Cucumis Sativus L.)是葫芦科甜瓜属蔬菜作物，在各个国家都有广泛种植。但在实际生产中由于外界条件例如低温弱光、湿度过大以及黄瓜种苗不佳等自身原因，导致黄瓜不正常结实、结畸形瓜、结果量少、甚至出现“化瓜”现象。通过研究黄瓜生长发育机理从而改善以上在实际生产中碰到的问题，达到提高黄瓜产量，改良黄瓜性状的目的。
黄瓜的果实发育是受多因素共同调控的复杂过程，其中Ca2+作为一种重要的第二信使，在其生长发育和胁迫响应的信号转导中发挥重要作用[1]。钙（Ca2+）信号传导系统依赖于钙感受器CaM/CML，CaM/CML作为钙信号系统传导下游的Ca2+受体，通过结合结构域与钙调素结合蛋白互作，从而促进整个钙信号系统传导的完成，调控植物的生理代谢及基因表达[2]。植物系统中各类钙感受器均为家族蛋白,目前对于CaM的研究较多，CML相对较少，仍有大部分钙感受器的生理作用及作用方法还未清楚。钙调素类似蛋白CML25-like调控花和果实发育的功能分析:http://www.751com.cn/shengwu/lunwen_24108.html