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外源6-BA对不结球紫色白菜中花青苷抗氧化性及相关酶活性的调控作用

时间:2018-07-26 21:20来源:毕业论文
花青苷是一种水溶性色素,广泛存在于植物的体内,是非常有潜力的天然色素的来源。因资源丰富、颜色鲜艳、无毒安全和具有抗氧化性等优势,花青苷的研究和开发利用备受关注。6

摘要:花青苷是一种水溶性色素,广泛存在于植物的体内,是非常有潜力的天然色素的来源。因资源丰富、颜色鲜艳、无毒安全和具有抗氧化性等优势,花青苷的研究和开发利用备受关注。6-BA是一种人工合成的细胞分裂素,不但在细胞生长和分化及其他相关的生理活动过程中起重要作用,还可以促进花青苷的积累,抑制或促进相关酶的活性。本实验以不结球白菜紫色品种NJX1-4和绿色品种苏州青为材料,利用外源6-BA处理植株,并测定不同时间点叶片中花青苷含量,抗氧化性及相关酶活性,探索6-BA对不结球白菜中花青苷的抗氧化性及相关酶活性的调控作用,为今后进一步研究花青苷的功能机制提供理论基础。26233
毕业论文关键词:花青苷;6-BA;抗氧化性;酶活性,不结球白菜
Effect of exogenous 6-BA on anthocyanins antioxidant activity and related enzyme activities in Purple non-heading Chinese Cabbage
Student majoring in Protected agriculture science and engineering    Chula Sa
 Tutor    Chunmei Hu
Abstract: Anthocyanins are water-soluble pigments which are widely found in plants, it is a potential source of natural pigments. The research and development of anthocyanins are important for its rich resources, bright colors, safe and antioxidant activity.6-BA is a synthetic cytokinin. It plays an important role in the cell growth and differentiation and other related physiological activities of the process. In addition, 6-BA can promote the accumulation of anthocyanins, as well as inhibit or induce other enzyme activity. In this study, we used two self-line of non-heading Chinese Cabbage: purple leaves NJX1-4 and green suzhouqing as the experimental materials. Leaves were treaded by exogenous 6-BA to explored the effect on anthocyanins antioxidant activity and related enzyme activities, providing a theoretical basis for further study of the functional mechanism of anthocyanins.
Key words: Anthocyanins; 6-BA; Antioxidant activity; Enzyme activity; non-heading Chinese Cabbage
目  录
摘要    3
关键词    3
Abstract    3
Key words    3
1  材料与方法    4
1.1  试验材料    5
1.2  试验方法    5
1.2.1  外源6-BA处理    5
1.2.2  总花青苷含量测定    5
1.2.3  总酚含量测定    5
1.2.4  抗氧化酶活性测定    5
1.2.5  叶片花青苷的抗氧化活性分析    6
1.3  数据分析    6
2  结果与分析    6
2.1  外源6-BA对不结球白菜中花青苷含量的影响    6
2.2  外源6-BA对不结球白菜叶片中总酚含量的影响    7
2.3  外源6-BA对不结球白菜叶片中相关酶活性的影响    8
2.3.1  外源6-BA处理对紫色不结球白菜中超氧化物歧化酶(SOD)活性的影响    8
2.3.2  外源6-BA处理对紫色不结球白菜中过氧化物酶(POD)活性的影响    8
2.3.3  源6-BA处理对紫色不结球白菜中过氧化氢酶(CAT)活性的影响    9
2.4  叶片抗氧化活性测定    9
3  讨论    10
3.1  外源6-BA处理不结球白菜后总花青苷含量和总酚及抗氧化能力的比较    10
3.2  外源6-BA处理对不结球紫色白菜中抗氧化酶的影响    10
3.3  外源6-BA处理对不结球紫色白菜中花青苷的抗氧化性影响    11 外源6-BA对不结球紫色白菜中花青苷抗氧化性及相关酶活性的调控作用:http://www.751com.cn/shengwu/lunwen_20337.html
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